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Optimization of optical tweezers for multimodal holographic microscope
Vaverka, Jan ; Antoš, Martin (referee) ; Dostál, Zbyněk (advisor)
This bachelor's thesis has described the principle of operation of interference microscopes with closer analysis of multimodal holograpfic microscope (MHM), which has been constructed at the Institut of Physical Engeneering (IPE) of Brno University of Technology. It has also analysed principles of optical trapping and has closely descirebed force effects on the trapped object with characteristic lenght bigger than wavelenght of trapping beam. It has designed optimization of existing optical tweezers module so that the module is connectable to the prototype of MHM, located at IPE. Simulation and optimization of the optical assembly of module have been performed, the mechanical design of module with the drawings has been designed. The module has been entered into production.
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Raman spectroscopy of prokaryotic cells
Večeríková, Paula ; Bernatová,, Silvie (referee) ; Samek, Ota (advisor)
The presented bachelor‘s thesis deals with the Raman spectroscopy of bacterial cells. This method proves to be very suitable for monitoring the culture conditions of these bacterial strains because it is essentially non-destructive and fast compared to gas chromatography. Due to it‘s non-destructive nature, it is possible to further cultivate the measured cells. This analytical method can also be used to sort and select individual cells with increased ability to produce PHB. This would enable the possibility to select the generation of so-called PHB superproducers. Polyhydroxybutyrate (PHB) belongs to the group of polyhydroxyalkanoates (PHA), which serve as a substitute for plastics made in the petrochemical industry, whose consumption is increasing, especially during a pandemic. Current biodegradable substitutes are based on starch, which, in turn, depletes nutritional material for humans and animals. This bachelor thesis can serve as a basis outline for reducing the cost of PHB production, because Raman spectroscopy can be used in cell culture as a sensor of PHB content in response to a bacterial strain. In the experimental part of the work, the Raman spectra of selected bacteria were measured, where the result showed the greatest response to PHB in Chelatococcus shambunathi and the lowest in the thermophilic isolate BZ (Paenibacillus sp.). Quantitative information on the content of PHA in bacteria was obtained by gas chromatography, where the highest content was the bacteria- Chelatococcus shambunathi and the lowest Termobacillus composti. The subject of the second part was the separation of a mixture of PHA-producing and non-PHA-producing bacterial cultures. The result clearly proves that the mixture is separable based on the intensity of the selected sorting parameter.
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Transitional motion analysis of the optically trapped particle in under-damped regime
Sűsserová, Martina ; Damková, Jana (referee) ; Harabiš, Vratislav (advisor)
The bachelor thesis deals with the analysis of the movement of optically trapped nanoparticles at low pressures and familiarizes with methods commonly used in these conditions. The practical part of this work was done in Matlab, where the undesirable signal interference was filtered out from the original data and their influence on the phase portraits of the acquired transients was analyzed. Furthermore, the damped oscillator parameters are obtained from the records of average trajectories and the distribution of position data during the transient compared with distribution of position data at stationary state by histograms.
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Stabilization of macroscopic particle in optical trap
Mlynář, Vojtěch ; Matěj, Zdeněk (referee) ; Brablc, Martin (advisor)
Cílem této práce je implementace a otestování algoritmů pro zpětnovazební stabilizaci částice v optické pasti. Tyto algoritmy jsou implementovány v programovatelném hradlovém poli Red Pitaya STEMlab 125-14 s využitím automatického generování kódu z modelů v programu Simulink. V práci je popsán fyzikální základ řízeného systému, jeho návaznost na teorii Kálmánova filtru, detaily implementovaných algoritmů v FPGA a procedury využité pro odhad parametrů. Implementované algoritmy byly úspěšně otestovány na reálné sestavě a výsledky jsou ukázány v závěru práce.
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Measurement of extinction spectra of optically trapped plasmon nano-particles
Flajšmanová, Jana ; Jonáš,, Alexander (referee) ; Brzobohatý, Oto (advisor)
This thesis deals with the dark-field imaging and the optical spectroscopy of optically trapped plasmonic nanoparticles. The optical trapping and the characterization of a single particle or multiple nanoparticles as well are demonstrated. The number of the optically trapped particles can be estimated from the dark-field scattering intensity. Experiments show the presence of the interparticle coupling among trapped metallic nanoparticles which has not been observed in case of dielectric particles. The scattering spectra of the plasmonic nanoparticles were compared with theoretical models based on the Mie theory and the Discrete dipole approximation.
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Controlling and Evaluation of Laser Micromanipulation Experiments
Kaňka, Jan ; Jákl, Petr (referee) ; Provazník, Ivo (advisor)
This work is focused on the development of a user friendly software interface using the LabViewTM environment that simplifies running of various experiments using laser micromanipulations and laser microspectroscopy of living microorganisms. Both techniques have been developing very fast for the last decade and belong to the growing group of contact-less and nondestructive techniques for manipulation and diagnostics of individual living microorganisms, cells, or viruses. Within this project we mastered the driving of peripheries, calibration of CCD scene, real-time image processing of the CCD scene, automatic selection of the cell for further laser processing, acquisition and processing of the Raman spectrum from living microorganisms. The final goal of our activity is fully automatic laser-based sorter of living cells depending on their chemical compositions. This work has been elaborated at the Institute of Scientific Instruments of the ASCR, v.v.i. under the supervision of prof. Pavel Zemanek.
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Correlation analysis of optically trapped particle motion
Koudelníček, Karel ; Jákl,, Petr (referee) ; Harabiš, Vratislav (advisor)
This bachelor’s thesis deals with the description of random (Brownian) motion of microscopic particle trapped by optical trap. This thesis provides overview of possible usage in biology. The major part of the thesis is dedicated to description of methods that allow characterize optical trap with etc. stiffness thanks to the random motion. First, the estimation methods are tested on the simulated data. Before using these methods for real data, it was necessary to modify them, specifically to eliminate crosstalk and frequency noise. The differences in the estimation of the stiffness from the autocorrelation function and the power spectrum were described in the conclusion.
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Behaviour of Objects in Structured Light Fields and Low Pressures
Flajšmanová, Jana ; Čižmár, Tomáš (referee) ; Marago, Onofrio (referee) ; Zemánek, Pavel (advisor)
Studium chování opticky zachycených částic nám umožňuje porozumět základním fyzikálním jevům plynoucím z interakce světla a hmoty. Předkládaná práce podává vysvětlení zesílení tažné síly působící na opticky svázané částice ve strukturovaném světelném poli, tzv. tažném svazku. Ukazujeme, že pohyb dvou opticky svázaných objektů v tažném svazku je silně závislý na jejich vzájemné vzdálenosti a prostorové orientaci, což rozšiřuje možnosti manipulace hmoty pomocí světla. Následně se práce zaměřuje na levitaci opticky zachycených částic ve vakuu. Představujeme novou metodologii na charakterizaci vlastností slabě nelinearního Duffingova oscilátoru reprezentovaného opticky levitující částicí. Metoda je založena na průměrování trajektorií s určitou počáteční pozicí ve fázovém prostoru sestávajícím z polohy a rychlosti částice a poskytuje informaci o parametrech oscilátoru přímo ze zaznamenaného pohybu. Náš inovativní postup je srovnán s běžně užívanou metodou založenou na analýze spektrální hustoty polohy částice a za využití numerických simulací ukazujeme její použitelnost i v nízkých tlacích, kde nelinearita hraje významnou roli.
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Optical tweezers for coherence-controlled holographic microscope
Straka, Branislav ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
In the master's thesis, there has been described and explained the principle of operation of the second generation coherence controlled holographic microscope (CCHM2) designed at the Brno University of Technology. There has also been listed theoretical description of the operation of the optical trap, together with the calculation of the forces acting on it, ways of measuring the stiffness of the optical trap and the principle of~creating a time-shared optical traps. The optical tweezers forming a separate module connectable to CCHM2 was designed. Simulation and optimization of parameters of the optical system, mechanical design, manufacturing documentation, current source to power the laser diode which allows to control the diode output power by the controller card connected to the PC was designed. The galvano-optics mirror angle is controlled by the PC card too. The optical tweezer has been designed, manufactured and tested in conjunction with the CCHM2.
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